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Altered proteome profiles in maternal plasma in pregnancies with fetal growth restriction

Haptoglobin α2 isoform as a potential biomarker

Clinical Proteomics volume 2, pages 169–184 (2006)Cite this article

Abstract

Fetal growth restriction (FGR) affects 3–5% of pregnancies and is associated with increased perinatal morbidity and mortality. Currently, there is no reliable biochemical test to differentiate a pathological FGR from a nonpathological one. The objective of this study was to screen whole maternal plasma to identify differentially expressed relatively abundant proteins associated with FGR. We analyzed maternal plasma from FGR (n=28) and healthy (n=22) pregnancies using two-dimensional gel electrophoresis (2D-GE) followed by software image analysis. Three spots with molecular weight (Mr) 18 kDa corresponding to haptoglobin (hp) α2, as identified by LC-MS/MS and immunoblotting, showed differential expression patterns in FGR. The distribution of hp α2 variants in maternal plasma samples showed the hp α2 variant 1 was low in 72% of FGR, medium in 16%, whereas high in 12%. In comparison, hp α2 variant 1 was high in (41%) of controls, medium in 41%, and low in 18% of cases. Based on the software image analysis, the mean spot volume for hp α2 variant 1 was 0.12 (SD=0.18) for FGR compared to 0.26 (SD=0.19) for control (p=0.006). Given that hp turnover is indicative of its maturation process and is traceable in plasma by its dominant/suppressed variants, we propose that hp α2 is an important potential target for evaluation of its clinical and pathophysiological role and as a diagnostic biomarker in FGR.

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Authors and Affiliations

  1. Department of Pediatrics, University of Western Ontario, 800 Commissioners Road E., N6C2V5, London, ON, Canada

    Madhulika B. Gupta, Maxim D. Seferovic & Victor K. M. Han

  2. Department of Biochemistry, University of Western Ontario, London, Canada

    Madhulika B. Gupta, Maxim D. Seferovic, Suya Liu, Amanda Doherty-Kirby, Gilles A. Lajoie & Victor K. M. Han

  3. Department of Obstetrics and Gynecology, University of Western Ontario, London, Canada

    Robert J. Gratton & Victor K. M. Han

  4. Children’s Health Research Institute, University of Western Ontario, London, Canada

    Madhulika B. Gupta, Maxim D. Seferovic, Robert J. Gratton, Gilles A. Lajoie & Victor K. M. Han

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  1. Madhulika B. Gupta
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Gupta, M.B., Seferovic, M.D., Liu, S. et al. Altered proteome profiles in maternal plasma in pregnancies with fetal growth restriction. Clin Proteom 2, 169–184 (2006). https://doiorg.publicaciones.saludcastillayleon.es/10.1007/BF02752499

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Clinical Proteomics

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