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Fig. 1 | Clinical Proteomics

Fig. 1

From: Optimized sample preparation and data analysis for TMT proteomic analysis of cerebrospinal fluid applied to the identification of Alzheimer’s disease biomarkers

Fig. 1

Experimental design to evaluate technical and total variation as well as an optimal normalization approach for our in-solution based TMT protocol. A pool of CSF from numerous individuals, referred to as Set 1 (A) or individual CSF samples consisting of 7 AD and 8 control samples, referred to as Set 2 (B) were split in 15 aliquots. Internal calibrator MSQCAL was spiked into each aliquot at equimolar amounts (50 fmol), while pepmix was added in differing amounts in triplicates (0, 10, 15, 20, 30 fmol). Each aliquot was subsequently TMT-labeled, combined, and analyzed by LC–MS

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Clinical Proteomics

ISSN: 1559-0275