Fig. 2

Cortical and medullary dissections from human kidney for LCM-microPOTS-TDP. (a) Shows a high-resolution image of the histological image taken post LCM processing for library production and dissection of glomeruli, tubules, and medullary rays. The inset region (i) represents the region of interest highlighted for (b), (c), and (d) where several glomeruli and tubules were dissected and the region is rotated within the zoomed visualization, where * denotes dissected glomeruli and ** denotes dissected tubules. The inset region (ii) represents the region of interest highlighted for (e), (f), and (g) where several areas of the medullary rays were dissected, where # denotes a medullary section which was dissected but not catapulted. (b) and (e) represent the autofluorescence image before LCM, (c) and (f) are the grayscale bright-field images before LCM, and (d) and (g) are the bright-field images after LCM. The scale bar for (b), (c), (d), (e), (f), and (g) is 250 μm