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Fig. 5 | Clinical Proteomics

Fig. 5

From: Spatial top-down proteomics for the functional characterization of human kidney

Fig. 5

Ion images from baseline resolved isotopes of intact proteoforms with IsoMatchMS annotations. The UniProt accession ID, amino acid sequence, PTMs, theoretical and observed m/z, and mass measurement error are reported for (a) cytochrome c oxidase subunit 7 C, mitochondrial, (b) large ribosomal subunit protein eL39, (c) small ribosomal subunit protein eS30, (d) truncated ubiquitin B, (e) intact ubiquitin B, (f) histone H4 acetylated and dimethylated (N-Ac/K20me2), (g) histone H4 diacetylated, (h) histone H4 diacetylated and dimethylated, (i) histone H2A type E, and (j) hemoglobin subunit A. The scale bar for (a) through (j) is 1 mm. (k) Shows a high-resolution image of the histological image taken post MALDI-MSI acquisition. Several hotspots can be noted off the tissue and were identified as embedding artifacts which did not influence analyses. SMART annotation: [43] S (step size, spot size, total pixels) = 40 μm, 12 μm x 15 μm, 86,189 pixels; M (molecular confidence) = MS1, < 5 ppm; A (annotations) = MS1 matching from LCM-microPOTS-TDP; R (resolving power) = 35k at m/z 11,306; T (time of acquisition) = 736 min.

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Clinical Proteomics

ISSN: 1559-0275